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STEDYCON

STEDYCON

Abberior Instruments is known for extraordinary customization ability of its flexible STED microscope platform. We have developed the ultimate compact STED and confocal microscope, called STEDYCON.

 

STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a versatile four-color confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system! At the same time, it is incredibly compact and can be used by anyone!

  • Convert your existing microscope - Instantly upgrade any epifluorescence microscope body to multicolor confocal and STED capabilities. The STEDYCON now has a variable pinhole and up to four detectors with superior DynamicPLUS Imaging. 
  • Get top-notch STED performance - With STEDYCON your microscope will image at 30nm resolution.
  • Save Space - The incredibly compact STEDYCON comes at the size of a camera.
  • Plug & Play - The STEDYCON is assembled within minutes. It simply connects to the C-mount camera port of your microscope with no need for optical alignment.
  • Service and maintenance free - The laser beams of the STEDYCON are aligned by design as provided by its patent-protected 'easySTED' optical arrangement.
  • Innovative web-based user interface - The STEDYCON can be controlled via a browser on any PC, MAC or even a tablet!

 

DynamicPLUS

Superior sensitivity and full dynamic range within the same image

Our new DynamicPLUS feature offers unrivaled performance with both STED and classical confocal applications. With DynamicPLUS, you can be sure to capture everything from the faintest details to the brightest spots.

The underlying avalanche photo detectors (APDs) have a superior quantum efficiency of up to 69% (@ 650nm) in the red fluorescence regime. This means that even when signal-levels are low, our APDs still collect plenty of photons for a meaningful image. Typical applications are super-resolution STED imaging and experiments with low labeling densities designed to stay close to physiological conditions.

At the same time, the dead-time compensation of DynamicPLUS allows crisp images of high-signal samples, such as bright confocal images. Of course, raw data for quantitative analysis and deconvolution is always available.

      

Our STEDYCON nanoscope works with all kinds of microscope bodies from all major vendors:

STEDYCON @ Leica DM2500
STEDYCON @ Leica DMI6000
STEDYCON @ Nikon NiE
STEDYCON @ Nikon Ti2
STEDYCON @ Nikon TiE
STEDYCON @ Olympus BX53
STEDYCON @ Olympus IX83
STEDYCON @ Zeiss Axio Imager
STEDYCON @ Zeiss Axio Observer with AFM
STEDYCON @ Zeiss Axio Observer Z1
STEDYCON @ Zeiss Axio Vert
Browser-based cross-platform software

The STEDYCON can be controlled via Abberior Instruments STEDYCON smart control, a web-browser-based graphical user interface. STEDYCON smart control was designed from scratch to provide the highest possible usability completing the overall STEDYCON concept of easy installation, intuitive usage, and maintenance-free operation. Beginners can image with super-resolution within minutes. Smart control runs on every device with a modern web browser like Windows-based PCs, Linux-based PCs, Apple PCs or even tablets.

   

Gattaquant STED-AFM ruler (70 nm distance between emitters) were imaged using a STEDYCON attached to a Nikon Eclipse Ni and a Zeiss Axio Imager Z2. Shown is RAW DATA

       

Videos

From zero to STED in no time 

Follow along with the incredibly quick installation of a STEDYCON super resolution microscope, uncut. We go from opening the carton to the first super-resolution STED image in under three minutes. 

Surface rendering of a confocal z-Stack of a pollen grain. Measured @ STEDYCON/ Nikon Ti2 body

Three color confocal z-Stack of mammalian cells. Z-plane distance: 250 nm. Labelled structures: Actin cytoskeleton (blue, Phalloidin, Oregon Green 488), Nuclear pore complex (green, NUP153, Abberior STAR 580), Mitochondria (green, Tom20, Abberior STAR 635P). Measured @ STEDYCON/ Zeiss body

Confocal microscopy stack acquired with the STEDYCON. Shown is 3 color sample with the actin cytoskeleton (blue) labelled with phalloidine-Abberior STAR488, Nuclear pore complex (Nup153) labelled with Abberior STAR RED and the Golgi protein (Giantin) labelled with Abberior STAR580.

Three color confocal z-Stack of pollen grains. Measured @ STEDYCON/ Nikon Ti2 body.

Further Information

  • Specs
    • Excitation laser lines: 405 nm (UV, cw, optional), 488 nm, 561nm, 640nm (all pulsed)
    • STED laser: 775nm (pulsed)
    • 2D STED resolution: <40nm, typically 30nm (depending on dyes and objectives used)
    • Easy-STED geometry: all laser lines aligned by design; maintenance free optical beam path
    • 3 confocal imaging channels
    • Detection: APD1: 650-700 nm; APD2: 575-625 nm; APD3: 505-545 nm; time-gated detection (for confocal and STED)
    • Upgradability to FLIM: get full FLIM functionality in all imaging channels through full compatibility with FLIM hardware from B+H or Picoquant
    • QUAD beam scanner: 90µm x 80µm (with a 100x objective); 512x512 pixel up to 1.1 frames/s; line frequency up to 800Hz
    • Pinhole: motorized pinhole with 12 different pinhole sizes
    • Software: browser-based control software operational on any modern PC, Mac or tablet: line interleaved STED and confocal imaging in three imaging channels; options to define z-stacks, acquisition queues and time series, auto-save function
    • Dimensions: STED-camera head: 11cm x 20cm x 20cm; controller: 22cm x 55cm x 60cm; total weight head plus controller unit < 40kg
    • STEDYCON is tested with virtually all microscope stands and objectives from all major vendors; contact us for details
    • Optionally: Optical table or antivibrational table, z-focusing piezo-drive, microscope stand and objective validated for STEDYCON

     

    The STEDYCON has been tested with the following configurations:

    • Zeiss Axio Imager Z2 (Upright) with objective 100x NA=1.46
    • Zeiss Axio Observer (Inverted) with objective 100x NA=1.46
    • Nikon NiE (Upright) with objective 100x NA=1.45 Lambda Series
    • Nikon TiE (Inverted) with objective 100x NA=1.45 Lambda Series
    • Olympus BX53 (Upright) with objective 100x NA=1.4
    • Olympus IX83 (Inverted) with objective 100x NA=1.4
    • Leica DMI6000 (Inverted) with objective 100x NA=1.4
    • Leica DM2500 (Upright) with objective 100x NA=1.4

     

    Please contact us to clarify the situation for your existing microscope.

  • Combinations
  • Downloads
  • Labels

    We recommend the following Abberior labels which are tested and optimized for Abberior superresolution systems to facilitate maximum resolution:

     

    > Overview of all Abberior labels