STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a versatile four-color confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system! At the same time, it is incredibly compact and can be used by anyone!
Superior sensitivity and full dynamic range within the same image
Our new DynamicPLUS feature offers unrivaled performance with both STED and classical confocal applications. With DynamicPLUS, you can be sure to capture everything from the faintest details to the brightest spots.
The underlying avalanche photo detectors (APDs) have a superior quantum efficiency of up to 69% (@ 650nm) in the red fluorescence regime. This means that even when signal-levels are low, our APDs still collect plenty of photons for a meaningful image. Typical applications are super-resolution STED imaging and experiments with low labeling densities designed to stay close to physiological conditions.
At the same time, the dead-time compensation of DynamicPLUS allows crisp images of high-signal samples, such as bright confocal images. Of course, raw data for quantitative analysis and deconvolution is always available.
Our STEDYCON nanoscope works with all kinds of microscope bodies from all major vendors:
The STEDYCON can be controlled via Abberior Instruments STEDYCON smart control, a web-browser-based graphical user interface. STEDYCON smart control was designed from scratch to provide the highest possible usability completing the overall STEDYCON concept of easy installation, intuitive usage, and maintenance-free operation. Beginners can image with super-resolution within minutes. Smart control runs on every device with a modern web browser like Windows-based PCs, Linux-based PCs, Apple PCs or even tablets.
From zero to STED in no time
Follow along with the incredibly quick installation of a STEDYCON super resolution microscope, uncut. We go from opening the carton to the first super-resolution STED image in under three minutes.
Surface rendering of a confocal z-Stack of a pollen grain. Measured @ STEDYCON/ Nikon Ti2 body
Three color confocal z-Stack of mammalian cells. Z-plane distance: 250 nm. Labelled structures: Actin cytoskeleton (blue, Phalloidin, Oregon Green 488), Nuclear pore complex (green, NUP153, Abberior STAR 580), Mitochondria (green, Tom20, Abberior STAR 635P). Measured @ STEDYCON/ Zeiss body
Confocal microscopy stack acquired with the STEDYCON. Shown is 3 color sample with the actin cytoskeleton (blue) labelled with phalloidine-Abberior STAR488, Nuclear pore complex (Nup153) labelled with Abberior STAR RED and the Golgi protein (Giantin) labelled with Abberior STAR580.
Three color confocal z-Stack of pollen grains. Measured @ STEDYCON/ Nikon Ti2 body.
Please contact us to clarify the situation for your existing microscope.