STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a versatile four-color confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system! At the same time, it is incredibly compact and can be used by anyone!
The STEDYCON now connects to motorized stages. For a full overview over even millimeter-sized samples, a tiled spiral scan is generated, which allows navigation and imaging within the whole context of your specimen. Subsequently, multiple regions of interest (ROI) can be placed in order to queue the automatic recording of many images with confocal or STED, while you go on and use the imaging time to prepare the next sample. Large ROIs are acquired as tile scan and can be easily stitched using SVI Huygens.
Our new push-button STEADYFOCUS module is a continuous laser-based hardware focus lock for the STEDYCON.
It guarantees confocal and STED imaging over days without focus drift.
On top, thanks to its proprietary design, it uniquely requires no additional optics in the imaging beam path, and therefore is 100% free of fluorescence losses or imaging distortions.
The STEADYFOCUS module is compatible with a multitude of immersion (water, oil, silicone, glycerol, ...) and embedding media (water-based, Mowiol, ...) and with upright and inverted microscope bodies of all major brands.
Examples of multicolor live-cell time-lapse confocal imaging using the STEADYFOCUS over many hours (up to 13 hrs).
Our fruitful collaboration with SVI has brought about cutting-edge superresolution at maximum convenience and benefit for our STEDYCON users.
As exemplified in the panels below, the combination of top-notch STED imaging with SVI Huygens’ sophisticated deconvolution algorithm reveals the faintest details in the sample. Moreover, all this unfolds at the push of a button!
Superior sensitivity and full dynamic range within the same image
Our new DynamicPLUS feature offers unrivaled performance with both STED and classical confocal applications. With DynamicPLUS, you can be sure to capture everything from the faintest details to the brightest spots.
The underlying avalanche photo detectors (APDs) have a superior quantum efficiency of up to 69% (@ 650nm) in the red fluorescence regime. This means that even when signal-levels are low, our APDs still collect plenty of photons for a meaningful image. Typical applications are super-resolution STED imaging and experiments with low labeling densities designed to stay close to physiological conditions.
At the same time, the dead-time compensation of DynamicPLUS allows crisp images of high-signal samples, such as bright confocal images. Of course, raw data for quantitative analysis and deconvolution is always available.
Our STEDYCON nanoscope works with all kinds of microscope bodies from all major vendors:
The STEDYCON can be controlled via Abberior Instruments STEDYCON smart control, a web-browser-based graphical user interface. STEDYCON smart control was designed from scratch to provide the highest possible usability completing the overall STEDYCON concept of easy installation, intuitive usage, and maintenance-free operation. Beginners can image with super-resolution within minutes. Smart control runs on every device with a modern web browser like Windows-based PCs, Linux-based PCs, Apple PCs or even tablets.
Three color confocal z-Stack of mammalian cells. Z-plane distance: 250 nm. Labelled structures: Actin cytoskeleton (blue, Phalloidin, Oregon Green 488), Nuclear pore complex (green, NUP153, Abberior STAR 580), Mitochondria (red, Tom20, Abberior STAR 635P). Measured @ STEDYCON/ Zeiss body
Please contact us to clarify the situation for your existing microscope.